Spectramax m series molecular devices pdf catalogs. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Spectramax 190 microplate spectrophotometer operators manual vii conventions used in this manual the names of keys that appear on the spectramax 190 control panel are shown in boxed helvetica type. Convert pdf to mp3 online and free this page also contains information on the pdf and mp3 file extensions. Molecular devices spectramax id3 multimode microplate reader. The spectramax 190 reader is the tool for dna analysis. Spectramax m2e multimode plate readers operators manual 01120115 rev. Spectramax microplate spectrophotometer operators manual. Spectramax m3 microplate reader from molecular devices llc. Uvvisible absorbance abs fluorescence intensity fi glow luminescence lumi the. Description the onboard microprocessor calculates and reports the absorbance, % transmittance, andor fluorescence for each well of a microplate or from the cuvette port. M5m5e mode reader customized to your specific applications or budgetary needs, while optional capabilities.
Spectramax m2 is similar to that of the gemini xps, a dedicated top read. The versamax microplate reader is built around a grating monochromator. Detection modes include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to. The spectramax m3 microplate reader is the standard for uvvisible multimode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6, 12, 24, 48, 96, and 384well microplates. The molecular devices spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability. How to use pdf viewer on new microsoft edge chromium. Molecular devices versamax microplate reader the molecular devices versamax elisa microplate reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequencefree assay flexibility. Spectramax plus 384 microplate reader manual conquer. The molecular devices spectramax 250 microplate reader has an optical design that mimics a dualbeam spectrophotometer. Molecular devices spectramax id3 multimode microplate. Molecular devices spectramax m2 multimode microplate reader.
The ability to detect 16 ngwell of dna and to quantitate 50 ng of dna provides the same sensitivity as a spectrophotometer. Spectramax m2m2e user guide university of michigan. Using the molecular devices test plate to calibrate the. The spectramax i3x multimode detection platform utilizes a patentpending design that allows for realtime system configuration by the user in less than two minutes. Molecular devices spectramax id3 multimode microplate reader special introductory offer.
The pathcheck sensor can extend the instrument linearity by a factor of three or more. Spectramax id5 multimode microplate reader youtube. Spectramax m5e multimode microplate reader molecular. The spectramax i3x reader allows one to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Spectramax m3 reader spectramax m4 reader spectramax m5 reader spectramax m5e reader detection modes. In this manual, we use the terms absorbance and optical density. The upgradeable spectramax m5 multimode microplate reader comes with the standard five modes of detection with the upgrade option to add htrf at a later time. Future ready the spectramax i3 x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. This revolution in modularity makes nocompromise detection the norm. Specifications see manual for details does uv and visible absorbance, and fluorescence intensity measurements on microplates and.
Accumulated intermediates, in the presence of reduced glutathione, are transported to the vacuoles, whereupon the development of the. Molecular devices spectramax m3 multi mode microplate reader. The spectramax m3 multimode microplate reader features dual monochromatic optics to allow the widest range of. Spectramax m3 reader spectramax m4 reader spectramax m5 reader spectramax m5e. Softmax pro software user guide marine biological laboratory. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96 or 384well microplate. Choose from a three m3, four m4, or five m5m5e mode reader customized to your specific applications or budgetary needs, while optional capabilities. Molecular devices spectramax id5 multimode microplate. Software for the test plate must be installed on the control computer before the plate can be used. Spectramax plus 384 microplate spectrophotometer operators manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices corporation service facility. Getting started how to use pdf viewer on new microsoft edge chromium yes, the new version of microsoft edge comes with a pdf reader, and these are the steps to get started. The following document is intended to demonstrate setup of this instrument. Detection modalities include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to a topoftheline dedicated spectrofluorometer or spectrophotometer. Spectramax m3, m4, m5, and m5e multimode microplate.
With optimized reagents, option to operate the readers. The spectramax m5e multimode microplate reader features a triplemode cuvette port, temperature control, microplate shaking, and the industryleading softmax pro. Molecular devices versamax microplate reader marshall. Molecular devices spectramax 250 microplate reader. The spectramax i3x multimode microplatereader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging and fast kinetics with injectors. This dual monochromatic, multi mode microplate reader delivers three modes of detection, uv vis absorbance reading, fluorescence and luminescence, and a broad spectrum of formats from cuvettes to 6, 12, 24, 48, 96, and 384well microplates. In addition, the id5 reader measures trf and fp and can be expanded to include trfret, htrf, bret, dual luciferase reporter assays with injectors, and western blot detection. The molecular devices spectramax m3 multimode microplate reader covers a wide range of applications without the need to change filters. For very concentrated samples, use a minimum volume. Spectramax plate reader eastern virginia medical school. Spectramax m3, m4, m5, and m5e multimode microplate reader. For fluorescence intensity assays, users can select from top or bottomread modes for improved sensitivity in solution and cellbased assays. Multimode microplate readers are a modular, upgradeable dualmonochromator microplate reader platform offering a. Spectramax m series multimode microplate readers molecular.
The cuvette chamber spectramax plus 384 only figure 1. Spectramax m3 multimode microplate reader, see page 122. The spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch. Each sample has a discrete sample and reference beam so that each well is measured directly. Spectramax m3, m4, m5, and m5e multimode microplate readers 01120115 f 9 pathcheck pathlength measurement technology a spectramax multimode microplate reader with pathcheck pathlength measurement technology allows normalization of variable well volumes to 1cm cuvette readings. In these mutants, intermediate metabolites of adenine biosynthesis pathway are accumulated. Spectramax and versamax plate readers operators manual 01120126 rev a 5 1. Robust, highvalue microplate readers can run samples based on predefined protocols and standard filter modes cover the entire visible range for a variety of assays. Molecular devices spectramax plus384 microplate reader manual attachments molecular devices spectramax plus384 microplate reader manual 624 kb. Spectramax id5 multimode microplate reader, a hybrid monochromator and filterbased, fivemode plate reader with automatic filter identification and western blot capability. All m series readers accept cuvettes and 6, 12, 24, 48, 96, and 384well microplates. Robust, highvalue microplate readers that can run samples based on predefined protocols and standard filter modes cover the entire visible range for a. Spectramax plus 384 microplate spectrophotometer operators manual introduction general overview the spectramax plus 384 microplate spectrophotometer provides rapid and sensitive uvvis measurements of a variety of analytes including speci. Pdf to mp3 convert your pdf to mp3 for free online.
These robust readers have been placed in labs from antarctica to the. Spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability. Multimode microplate reader spectra max m3 climate impacts. Pdf is a file format developed by adobe systems for representing documents in a manner that is separate from the original operating system, application or hardware from where it was originally created. Spectramax plus 384 microplate reader molecular devices. The spectramax plus 384 microplate reader from molecular devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Spectramax m3 multimode microplate reader, molecular devices. These documents are in pdf format and can be viewed using a pdf viewer like. The spectramax m series multimode microplate readers measure uv and visible absorbance, fluorescence, luminescence, fluorescence polarization, trf and htrf. The spectramax m3, m4, m5, and m5e microplate readers are a series. This easy to use multimode plate reader is designed to handle the work flow of multiple research projects. The spectramax id3 and id5 multimode microplate readers measure absorbance, fluorescence, and luminescence.
The spectramax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Spectramax id3 and id5 hybrid multimode microplate reader. With optimized reagents and the industryleading data acquisition and. Spectramax m3, m4, m5, and m5 e multimode microplate readers 01120115 f 9 pathcheck pathlength meas urement technology a spectramax multimode microplate reader with pathcheck pathlength measurement technology allows normalization of variable well volumes to 1cm cuvette readings. If the plate is new, or has been recertified since the last calibration, the new absorbance values must. Spectramax id3 and id5 multimode microplate readers.
Insert your plate into the blue adapter, making sure it is lying flat inside the clips. Spectramax i3x multimode microplate reader molecular. Spectramax m series multimode microplate readers biothing. Located at the right front of the spectramax plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette.
Turn on the plate reader with the rocking toggle switch on the back righthand side. Spectramax id3 multimode microplate reader molecular. Using the molecular devices test plate to calibrate the spectramax reader. Spectramax m3, m4, m5, and m5 e multimode microplate readers.